ABSTRACT

Concurrent Administration of the Methanol Leaf Extract of Leptadenia Hastata (Pers) Decne (Apocynaceae) With Metformin or Glibenclamide Influences Blood Glucose Handling in Normal Rats
Omobhude F. Aluefua, Aminu Chika, Aminu Ishaka, Kabiru Abubakar
Background: Leptadenia hastata (Pers) Decne (Apocynaceae) is a familiar medicinal plant utilized in northern Nigeria either singly or together with conventional drugs to treat diabetes mellitus. Objective: This study investigated the influence of concurrent administration of the methanol leaf extract of L. hastata with Metformin or Glibenclamide on blood glucose handling in normal male Wister rats. Methods: One hundred and twenty-eight normal rats were randomized into 16 groups of 8 rats each. The first group served as a vehicle administered control. Three groups were treated with three increasing doses of the extract using a semilogarithmic scale (50mg/kg, 150mg/kg and 500mg/kg respectively). Another three groups were treated with Glibenclamide at doses of 0.3mg/kg, 1mg/kg and 3mg/kg respectively. Another three groups were treated with metformin at doses of 30mg/kg, 100mg/kg, and 300mg/kg respectively. Another three groups were treated with metformin (100mg/kg) co-administered with the extract at 50mg/kg, metformin 100mg/kg and the extract at 150mg/kg, metformin 100mg/kg and the extract at 500mg/kg respectively. The last three groups received Glibenclamide at 1mg/kg co-administered with the methanol extract at 50mg/kg, Glibenclamide at 1mg/kg with the extract at 150mg/kg, and Glibenclamide 1mg/kg with the extract at 500mg/kg respectively. Oral glucose tolerance test (OGTT) was conducted following 8 hours of fasting. Results: The result revealed a significant reduction in the total area under the glucose tolerance curve when metformin was co-administered with the extract in normal rats. Conclusion: The findings suggest that the methanol leaf extract of L. hastata when co-administered with metformin caused an enhancement of the effect of metformin following OGTT in normal rats. Keywords: Blood glucose, extract, metformin, glibenclamide, glucose tolerance.
10.51658/ABMS.202232.4
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